A Low Cost Metal-Free Vascular Access Mini-Port for Artifact Free Imaging and Repeated Injections in Mice

نویسندگان

  • Teresa Fiebig
  • Giovanna Figueiredo
  • Hanne Boll
  • Hans Ulrich Kerl
  • Ingo S. Noelte
  • Alex Forster
  • Christoph Groden
  • Martin Kramer
  • Marc A. Brockmann
چکیده

PURPOSE Small injection ports for mice are increasingly used for drug testing or when administering contrast agents. Commercially available mini-ports are expensive single-use items that cause imaging-artifacts. We developed and tested an artifact-free, low-cost, vascular access mini-port (VAMP) for mice. PROCEDURES Leakage testing of the VAMP was conducted with high speed bolus injections of different contrast agents. VAMP-induced artifacts were assessed using a micro-CT and a small animal MRI (9.4T) scanner ex vivo. Repeated contrast administration was performed in vivo. RESULTS With the VAMP there was no evidence of leakage with repeated punctures, high speed bolus contrast injections, and drawing of blood samples. In contrast to the tested commercially available ports, the VAMP did not cause artifacts with MRI or CT imaging. CONCLUSIONS The VAMP is an alternative to commercially available mini-ports and has useful applications in animal research involving imaging procedures and contrast agent testing.

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عنوان ژورنال:

دوره 8  شماره 

صفحات  -

تاریخ انتشار 2013